Absolute Eosinophil Count is actually a diagnostic test used for the quantitative estimation of Absolute Eosinophil Count in the human body using Hemocytometer. Abnormality in the number of Eosinophil cells indicates some abnormality in the body.
Increased number of eosinophil indicates a variety of conditions like allergy, parasitism, drug reaction, collage vascular diseases, leukemia. Decreased number of eosinophil indicates conditions like Cushing’s syndrome, hyper-adrenalism.
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How Absolute Eosinophil Count Test is Done?
The whole blood sample is collected and it is diluted with Eosinophil Diluting Fluid for lysing off RBCs, other types of leukocytes except Eosinophil which appear in red color because of Eosin stain. Such remaining cells are counted by using Neubauer Chamber using low power microscope (10x).
How is Sample Collected for Absolute Eosinophil Count Test?
- Whole blood should be collected as a sample
- 2 mL of blood should be collected into a K2 EDTA vacutainer
- The specimen should be processed immediately within 2 hours of collection
- The blood sample should be mixed gently for at least 2 minutes by keeping the tube in between the palms of hands before testing.
- Clotted and contaminated samples should NOT be used.
Chemicals / Reagents / Equipment Required for Absoulte Eosionphil Count Test?
Light microscope, Neubauer chamber, pipettes, glass test tubes, and micropipettes are the equipment required for this test which also requires the following chemicals:
Eosinophil Diluting fluid i.e. Hingleman’s Solution prepared by:
- Distilled Water – 99 mm
- Formalin – 0.5 ml
- 95% phenol – 0.5 ml
- Yellow Eosin – 0.5 g
What is the Procedure of Absolute Eosinophil Count Test?
For performing Absolute Eosinophil Count Test in a standard procedure, following the step-by-step guide is useful:
- Pipette 0.36 mL Eosinophil diluting fluid in a test tube
- Add 0.04 mL of K2 EDTA blood
- Mix well for just 30 seconds and kept intact for 10 minutes
- Charge the Neubauer Counting Chamber
- Keep it in a moist chamber for at least 2 to 3 minutes
- Count the cells under low power objective with reduced light in all the squares of Neubauer chamber.
How to Calculate Absolute Eosinophil Count?
After performing the above mentioned procedure, the following calculations are done to arrive at the total number of eosinophil in the body, this is the universal Absolute Eosinophil Count Formula:-
Total no. of Eosinophils, cu.mm (µl) = No. of cells counted X Dilution / Volume of fluid
== > No. of cells counted x 10 / 0.9
Dilution = 10
Volume of fluid = area counted x depth = 9 sq.mm x 0.1 = 0.9
Results calculated according to the above formula are reported as number of eosinophil per cu.mm of whole blood.
Precautions to be Taken During Absolute Eosinophil Count Test
- Counting chamber should be clean and dry
- Ensure air bubbles DON’T enter while pipetting or diluting
- Counting chamber should be loaded in one go
- Make sure that fluid doesn’t overrun the equipment
- Thick and flat coverslip should be employed
- Start counting the cells after giving at least 2 to 3 minutes for the cells to settle
- Include all the cells lying within the square and also below the lines or borders
- Handle samples with care because they can be potentially infectious
- Handle reagents with much care and avoid their contact with eyes, skin, and mouth
- Mouth pipetting should NOT be performed
- Standard disposal procedure should be followed for disposing of the used chemicals and samples
What are the potential sources of variability in Absolute Eosinophil Count Test?
Chances of error or potential sources of variability in Absolute Eosinophil Count Test include:
- Eosinophil cells tend to disintegrate in diluting fluid. For avoiding this problem, counting should be done within 30 minutes of diluting the sample.
Absolute Eosinophil Count Test FAQs
What is the Normal / Standard / Reference Range for Absolute Eosinophil Count Test?
The normal range of results: 40 to 440 cells / cu.mm of whole blood
What is a normal absolute eosinophil count?
An absolute eosinophil count is done through blood tests to measure the number of white blood cells. These cells become active when one have allergic diseases, infections or other medical disorders. (1). The normal value of the eosinophil count is 600cells /microliter. (2). Based on the count the doctors determine the disease if there are no outward symptoms and prescribe the medications.
What is the Absolute Eosinophil Count Unit?
The unit of the count is cells/ microliter. To calculate the absolute eosinophil count blood, samples are drawn from the body and tested. While computing the absolute eosinophil count the eosinophil percentage and the white blood cell counts are used by the calculator, and an estimation of the acidophilic cells in the blood is measured.
What would cause absolute high absolute eosinophil count?
High absolute eosinophil count occurs when there are endocrine disorders, fungal and parasitic diseases, skin disorders, allergies due to food or medications, autoimmune disorders, tumors and toxins present in the body. They can also be due to cancer, leukemia, idiopathic hypereosinophilic syndrome or HES the cause of which is not known and many other infections and Hodgkin’s disease. (3)
How to naturally reduce absolute eosinophil count?
One can try to reduce the absolute eosinophil count by changing the lifestyle and reducing stress, changing the food habit and eating less acidic foods, staying away from known allergens, consuming ginger to control the inflammations and using turmeric in cooking. Drinking basil (Tulsi) juice, inhaling the smell of eucalyptus oil, mulethy tea, eating coriander seeds with rice water and sugar helps to reduce the count naturally. (4)
What is considered a high absolute eosinophil count?
The eosinophil count more than 500 cells/microliter is considered to be high absolute eosinophil count although this level may vary from laboratory to laboratory and methods applied for testing. However, any result of the blood test where the count is above 500 cells/ microliter is alarming and one needs to consult a physician for the remedy.
What does absolute eosinophil count of 1.0 mean?
The result which indicates the absolute eosinophil count as 1.0 after the blood test signifies the there is a presence of high white blood cells and some infection has attacked the body. It generally represents some blood-related disease and may turn to hematological malignancy if not treated with importance t reduce the levels. (5)
Absolute eosinophil count in females?
The absolute eosinophil count does not vary with gender. Studies have proved that the count level is irrespective of gender and the normal absolute eosinophil count for females remains the same as of the males. (6). The test is done to measure the number of white blood cells. These cells become active when one have allergic diseases, infections or other medical disorders. (7)
Absolute Eosinophil Count Uses
The absolute eosinophil count is measured to determine the underlying health conditions by measuring the white blood cell count in the blood. (8) They are used to determine if any allergy or infection is present in the blood or any tumors or cancer is nesting in the body. (9).
How much is the absolute eosinophil count during pregnancy?
The absolute eosinophil count during pregnancy does not change as of the regular time in a woman, and if there is any change recorded during pregnancy then it is to be understood that the pregnant woman is having some infections irrespective of her being pregnant. The count should be 0 to 600 cells/ microliter during pregnancy as well as normal times.
How much is absolute eosinophil count in a child?
The eosinophil count in a child should be around 350 cells/ microliter, and it is done by taking blood samples from the body. The count represents the number of white blood cells and the presence of acidophilic cells in the body. (10)
What does absolute eosinophil count is zero mean?
When the levels of absolute eosinophil count are zero, it represents that there is no disorder and the scale is within the normal range. Any standard less than 0 and higher than 600 cells/microliter should be brought to the notice of the physician for the correct treatment of some underlying ailment.
How much is the absolute eosinophil count for nucala?
The absolute eosinophil count for patients with severe asthma is over 600 cells/microliter, and Nucala helps to reduce the excess count above 600 to normal levels. Nucala is the medication used by severe asthma patients to breathe well and not used for sudden breathlessness. (11)
What does absolute eosinophil count is 800?
If anyone is having an absolute eosinophil count of 800 cells/ microliter, it indicates the person is suffering from allergic or blood infections. It can occur when there are endocrine disorders, fungal and parasitic diseases, skin disorders, allergies due to food or medications, autoimmune disorders, tumors and toxins present in the body. They can also be due to cancer, leukemia, idiopathic hypereosinophilic syndrome or HES the cause of which is not known and many other infections and Hodgkin’s disease.